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KMID : 0352519980350030218
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Korea Univercity Medical Journal
1998 Volume.35 No. 3 p.218 ~ p.227
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Expression of Vascular Endothelial Growth Factor in Normal and Diseased Human Inferior Turbinate, Ethmoid Sinus Mucosa and Nasal Polyp
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Abstract
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Vascular permeability factor (VPF) , also known as vascular endothelial growth factor(VEGF) and vasculotropin is a potent multifunctional cytokine that exerts several important and possibly independent actions on vascular endothelium. VEGF was originally discovered as a tumor-secreted protein that rendered venules and small veins hyperpermeable to circulating macromolecules. Subsequently there were some evidences that VEGF acts directly on cultured endothelial cells to induce transient accumulation of cytoplasmic calcium, change of shape. cell division and migration. In addition, VEGF alters the pattern of endothelial cells gene expression and induces angiogenesis in vivo. Nasal mucosa is known to be highly permeable tissue under the normal physiological condition. Chronic inflammatory nasal diseases tend to increase the vascular permeability of nasal mucosa , including the edematous nasal mucosa and nasal polyp.
In this study, we tried to confirm the presence of VEGF protein and its mRNA in normal and diseased nasal mucosa by immunohistochemistry, western blotting and in situ hybridization. Immunohistochemical reaction for anti-VEGF protein was seen in endothelial cells lining various sized vessels, and muscular cells surrounding the cavernous sinusoidal vessels of normal inferior turbinate mucosa In allergic inferior turbinate mucosa and nasal polyps, inflammatory cells showed the positive reaction in allergic inferior turbinate mucosa . In addition to these findings, myofibroblast dispersed
in tissue was observed in nasal polyp and edematous ethmoid mucosa. Western blotting showed also the presence of VEGF protein of expected size in each sample, Hybridization of an antisense VEGF probe was concentrated in normal inferior turbinate, allergic inferior turbinate, normal ethmoid mucosa, edematous ethmoid mucosa and nasal polyp. These results suggest that VEGF may play important roles in regulation of nasal vascular permeability and formation of edematous nasal mucosa in chronic inflammation.
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KEYWORD
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Vascular endothelial growth factor, Human nasal mucosa, Nasal polyp
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